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產品名稱：重組人類巨噬細胞炎性蛋白質3α/ CCL20蛋白
產品型號：rHuMIP-3α/CCL20
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組人類巨噬細胞炎性蛋白質3α/ CCL20蛋白與其它公司提供的重組蛋白不同，rHuMIP-3α/CCL20蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人類巨噬細胞炎性蛋白質3α/ CCL20蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組人類巨噬細胞炎性蛋白質3α/ CCL20蛋白

Synonyms LARC, Beta-chemokine Exodus-1, Liver and Activation-regulated Chemokine

Species Human

Accession P78556

GeneID 6364

Source 重組人類巨噬細胞炎性蛋白質3α/ CCL20蛋白Escherichia coli.

Molecular Weight Approximately 8.0 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids.

Quantity 5μg/20μg/1000μg

AA Sequence ASNFDCCLGY TDRILHPKFI VGFTRQLANE GCDINAIIFH TKKKLSVCAN PKQTWVKYIV RLLSKKVKNM

Purity > 97 % by SDS-PAGE and HPLC analyses.

Biological Activity 重組人類巨噬細胞炎性蛋白質3α/ CCL20蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 10-50 ng/ml.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.

Endotoxin Less than 1 EU/μg of rHuMIP-3α/CCL20 as determined by LAL method.

Reconstitution 重組人類巨噬細胞炎性蛋白質3α/ CCL20蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組人類巨噬細胞炎性蛋白質3α/ CCL20蛋白

SDS-PAGE

Reference 1. Chavan R, Marfatia KA, An IC, et al. 2006. J Virol, 80: 7676-87.
2. Hosokawa Y, Hosokawa I, Ozaki K, et al. 2005. Clin Exp Immunol, 142: 285-91.
3. Hirota K, Yoshitomi H, Hashimoto M, et al. 2007. J Exp Med, 204: 2803-12.
4. Francis JN, Sabroe I, Lloyd CM, et al. 2008. Clin Exp Immunol, 152: 440-7.

Background CCL20, also named LARC (Liver and Activation-regulated Chemokine), is encoded by CCL20 located on chromosome 2 in humans. The expression can be induced by LPS and some inflammatory cytokines, and it is at a high level in lymph nodes, PBL, fatal liver, fatal lung, appendix and so on. CCL20 has functions of chemotactic towards lymphocytes and dendritic cells. Additionally, it promotes the adhesion of memory CD4+ T cells and inhibits colony formation of bone marrow myeloid immature progenitors. CCL20 elicits it effects by binding with CCR6 receptor.

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Synonyms	LARC, Beta-chemokine Exodus-1, Liver and Activation-regulated Chemokine
Species	Human
Accession	P78556
GeneID	6364
Source	重組人類巨噬細胞炎性蛋白質3α/ CCL20蛋白Escherichia coli.
Molecular Weight	Approximately 8.0 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids.
Quantity	5μg/20μg/1000μg
AA Sequence	ASNFDCCLGY TDRILHPKFI VGFTRQLANE GCDINAIIFH TKKKLSVCAN PKQTWVKYIV RLLSKKVKNM
Purity	> 97 % by SDS-PAGE and HPLC analyses.
Biological Activity	重組人類巨噬細胞炎性蛋白質3α/ CCL20蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 10-50 ng/ml.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.
Endotoxin	Less than 1 EU/μg of rHuMIP-3α/CCL20 as determined by LAL method.
Reconstitution	重組人類巨噬細胞炎性蛋白質3α/ CCL20蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組人類巨噬細胞炎性蛋白質3α/ CCL20蛋白
SDS-PAGE
Reference	1. Chavan R, Marfatia KA, An IC, et al. 2006. J Virol, 80: 7676-87. 2. Hosokawa Y, Hosokawa I, Ozaki K, et al. 2005. Clin Exp Immunol, 142: 285-91. 3. Hirota K, Yoshitomi H, Hashimoto M, et al. 2007. J Exp Med, 204: 2803-12. 4. Francis JN, Sabroe I, Lloyd CM, et al. 2008. Clin Exp Immunol, 152: 440-7.
Background	CCL20, also named LARC (Liver and Activation-regulated Chemokine), is encoded by CCL20 located on chromosome 2 in humans. The expression can be induced by LPS and some inflammatory cytokines, and it is at a high level in lymph nodes, PBL, fatal liver, fatal lung, appendix and so on. CCL20 has functions of chemotactic towards lymphocytes and dendritic cells. Additionally, it promotes the adhesion of memory CD4+ T cells and inhibits colony formation of bone marrow myeloid immature progenitors. CCL20 elicits it effects by binding with CCR6 receptor.

產品名稱：重組人類巨噬細胞炎性蛋白質3α/ CCL20蛋白