產(chǎn)品詳情
簡(jiǎn)單介紹：
重組人膠質(zhì)細(xì)胞系衍生的神經(jīng)營(yíng)養(yǎng)因子蛋白與其它公司提供的重組蛋白不同，rHuGDNF蛋白產(chǎn)品為采用CFS的無(wú)細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來(lái)的重組蛋白，可表達(dá)出對(duì)細(xì)胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時(shí)獨(dú)有的全自動(dòng)蛋白純化技術(shù)則簡(jiǎn)便高效，將蛋白純化過(guò)程中對(duì)蛋白的損傷降低到*小程度.重組人膠質(zhì)細(xì)胞系衍生的神經(jīng)營(yíng)養(yǎng)因子蛋白（全長(zhǎng)序列）產(chǎn)品可用于Western Blot驗(yàn)證、抗體制備、蛋白檢測(cè)、ELISA等試驗(yàn)中.
詳情介紹：
重組人膠質(zhì)細(xì)胞系衍生的神經(jīng)營(yíng)養(yǎng)因子蛋白
| Synonyms | ATF, ATF-1 |
| Species | Human |
| Accession | P39905 |
| GeneID | 2668 |
| Source | Escherichia coli. |
| Molecular Weight | 重組人膠質(zhì)細(xì)胞系衍生的神經(jīng)營(yíng)養(yǎng)因子蛋白Approximately 30.1 kDa, a homodimeric protein consisting of two 134 amino acid non-glycosylated polypeptide chains. |
| Quantity | 2μg/10μg/1000μg |
| AA Sequence | SPDKQMAVLP RRERNRQAAA ANPENSRGKG RRGQRGKNRG CVLTAIHLNV TDLGLGYETK EELIFRYCSG SCDAAETTYD KILKNLSRNR RLVSDKVGQA CCRPIAFDDD LSFLDDNLVY HILRKHSAKR CGCI |
| Purity | 重組人膠質(zhì)細(xì)胞系衍生的神經(jīng)營(yíng)養(yǎng)因子蛋白> 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 5.0 × 107 IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | 重組人膠質(zhì)細(xì)胞系衍生的神經(jīng)營(yíng)養(yǎng)因子蛋白Lyophilized from a 0.2 μm filtered concentrated solution in 1 × PBS, pH 7.4, with 0.05 % Tween-20. |
| Endotoxin | Less than 0.1 EU/μg of rHuGDNF as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人膠質(zhì)細(xì)胞系衍生的神經(jīng)營(yíng)養(yǎng)因子蛋白 | |
| SDS-PAGE |
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| Reference |
1. Wood MD, Kim H, Bilbily A, et al. 2012. Muscle Nerve, 46: 122-4. 2. Stahl K, Mylonakou MN, Skare O, et al. 2011. Brain Res, 1378: 105-18. 3. Cornejo M, Nambi D, Walheim C, et al. 2010. Neurochem Res, 35: 1643-51. 4. Boado RJ, Zhang Y, Wang Y, et al. 2008. Biotechnol Bioeng, 100: 387-96. |
| Background | Glial cell-derived neurotrophic factor is a neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. It is a founding member of the GDNF family of ligands (GFL) and has been shown to interact with GFRA2 and GDNF family receptor alpha. GDNF (monomer) contains seven conserved cysteine residues, one of which (Cys 101) is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration. Rat and human mature GDNF shows 93 % sequence identity and have species cross-reactivity. |
