產(chǎn)品詳情
簡單介紹：
重組人白細(xì)胞介素-6蛋白與其它公司提供的重組蛋白不同，rHuIL-6蛋白產(chǎn)品為采用CFS的無細(xì)胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細(xì)胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術(shù)則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人白細(xì)胞介素-6蛋白（全長序列）產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人白細(xì)胞介素-6蛋白
| Synonyms | BSF-2, CDF, Hybridoma growth factor, IFN-beta-2. |
| Species | Human |
| Accession | P05231 |
| GeneID | 3569 |
| Source | Escherichia coli. |
| Molecular Weight | 重組人白細(xì)胞介素-6蛋白Approximately 20.7 kDa, a single non-glycosylated polypeptide chain containing 183 amino acids. |
| Quantity | 5μg/20μg/1000μg |
| AA Sequence | VPPGEDSKDV AAPHRQPLTS SERIDKQIRY ILDGISALRK ETCNKSNMCE SSKEALAENN LNLPKMAEKD GCFQSGFNEE TCLVKIITGL LEFEVYLEYL QNRFESSEEQ ARAVQMSTKV LIQFLQKKAK NLDAITTPDP TTNASLLTKL QAQNQWLQDM TTHLILRSFK EFLQSSLRAL RQM |
| Purity | > 96 % by SDS-PAGE and HPLC analyses. |
| Biological Activity |
重組人白細(xì)胞介素-6蛋白Assay #1: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using IL-6-dependent murine 7TD1 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107 IU/mg. Assay #2: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using IL-6-dependent murine T1165 cells is less than 0.8 ng/ml, corresponding to a specific activity of > 1.25 × 106 IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | 重組人白細(xì)胞介素-6蛋白Less than 1.0 EU/μg of rHuIL-6 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人白細(xì)胞介素-6蛋白 | |
| SDS-PAGE |
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| Reference |
1. Ferguson-Smith AC, Chen YF, Newman MS, et al. 1988. Genomics. 2:203-8. 2. van der Poll T, Keogh CV, Guirao X, et al. 1997. J Infect Dis. 176:439-44. 3. Ming JE, Cernetti C, Steinman RM, et al. 1989. J Mol Cell Immunol. 4:203-11; discussion 211-2. 4. Bastard JP, Jardel C, Delattre J, et al. 1999. Circulation. 99:2221-2. 5. Heinrich PC, Behrmann I, Muller-Newen G, et al. 1998. Biochem J. 334 ( Pt 2):297-314. |
| Background | Interleukin-6 (IL-6) is an interleukin that in humans is encoded by the IL-6 gene and acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response. Furthermore, It plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It also induces myeloma and plasmacytoma growth and induces nerve cells differentiation acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. The human IL-6 is a single non-glycosylated polypeptide chain containing 183 amino acids and it signals through a cell-surface type I cytokine receptor complex consisting of the ligand-binding IL-6Rα chain (CD126), and the signal- transducing component gp130 (also called CD130). The human IL-6 shares about 40% a.a. sequence identity with mouse and rat IL-6 and it is equally active on mouse and rat cells. |
