產品詳情
簡單介紹：
重組人粘膜相關上皮趨化因子/ CCL28蛋白與其它公司提供的重組蛋白不同，rHuMEC/CCL28蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人粘膜相關上皮趨化因子/ CCL28蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人粘膜相關上皮趨化因子/ CCL28蛋白
| Synonyms | CCK1 |
| Species | Human |
| Accession | Q9NRJ3 |
| GeneID | 56477 |
| Source | Escherichia coli. |
| Molecular Weight | 重組人粘膜相關上皮趨化因子/ CCL28蛋白Approximately 12.4 kDa, a single non-glycosylated polypeptide chain containing 108 amino acids. |
| Quantity | 5μg/20μg/1000μg |
| AA Sequence | SEAILPIASS CCTEVSHHIS RRLLERVNMC RIQRADGDCD LAAVILHVKR RRICVSPHNH TVKQWMKVQA AKKNGKGNVC HRKKHHGKRN SNRAHQGKHE TYGHKTPY |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組人粘膜相關上皮趨化因子/ CCL28蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human lymphocytes is in a concentration range of 1.0-10.0 ng/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 130 mM NaCl. |
| Endotoxin | Less than 1 EU/μg of rHuMEC/CCL28 as determined by LAL method. |
| Reconstitution | 重組人粘膜相關上皮趨化因子/ CCL28蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人粘膜相關上皮趨化因子/ CCL28蛋白 | |
| SDS-PAGE |
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| Reference |
1. Wang W, Soto H, Oldham ER, et al. 2000. J Biol Chem, 275: 22313-23. 2. Hieshima K, Ohtani H, Shibano M, et al. 2003. J Immunol, 170: 1452-61. 3. Eksteen B, Miles A, Curbishley SM, et al. 2006. J Immunol, 177: 593-603. 4. Kagami S, Kakinuma T, Saeki H, et al. 2005. J Invest Dermatol, 124: 1088-90. |
| Background | CCL28 belongs to CC family chemokine, also named mucosae-associated epithelial chemokine (MEC). The CCL28 cDNA is located on Chr.5 in humans, and the mRNA of CCL28 is highly expressed by epithelial cells of diverse tissues including normal and pathological colon, salivary gland, mammary gland, trachea and rectum. The amino acids sequence of human and mouse CCL28 shares 83 % identity, when the protein is processed to be the mature form. CCL28 is signaling through CCR3 and CCR10, and chemotactic for resting CD4, CD8 T-cells and eosinophils. |
