產品詳情
簡單介紹：
重組人白細胞介素-8,79a.a./CXCL8蛋白與其它公司提供的重組蛋白不同，rHuIL-8, 72a.a./CXCL8蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人白細胞介素-8,79a.a./CXCL8蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人白細胞介素-8,79a.a./CXCL8蛋白
| Synonyms | (Ser-IL-8)72, GCP/IL-8 protein I, IL8/NAP1 form III, LYNAP, MDNCF-c, NAF |
| Species | Human |
| Accession | P10145 |
| GeneID | 3576 |
| Source | 重組人白細胞介素-8,79a.a./CXCL8蛋白Escherichia coli. |
| Molecular Weight | Approximately 8.4 kDa, a single non-glycosylated polypeptide chain containing 72 amino acids. |
| Quantity | 5μg/25μg/1000μg |
| AA Sequence | SAKELRCQCI KTYSKPFHPK FIKELRVIES GPHCANTEII VKLSDGRELC LDPKENWVQR VVEKFLKRAE NS |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組人白細胞介素-8,79a.a./CXCL8蛋白Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human CXCR2 transfected mouse BaF3 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/μg of rHuIL-8, 72a.a./CXCL8 as determined by LAL method. |
| Reconstitution | 重組人白細胞介素-8,79a.a./CXCL8蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人白細胞介素-8,79a.a./CXCL8蛋白 | |
| SDS-PAGE |
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| Reference |
1. Modi WS, Dean M, Seuanez HN, et al. 1990. Hum Genet. 84:185-7. 2. Wolff B, Burns AR, Middleton J, et al. 1998. J Exp Med. 188:1757-62. 3. Utgaard JO, Jahnsen FL, Bakka A, et al. 1998. J Exp Med. 188:1751-6. 4. Van Damme J, Rampart M, Conings R, et al. 1990. Eur J Immunol. 20:2113-8. |
| Background | Interleukin-8 (IL-8) is encoded by the IL8 gene andproduced by macrophages and other cell types such as epithelial cells. It is also synthesized by endothelial cells, which store IL-8 in their storage vesicles. There are many receptors capable to bind IL-8, the most affinity to IL-8 are receptors CXCR1, and CXCR2. As a member of the CXC chemokine family, function of IL-8 is the induction of chemotaxis in its target cells, like neutrophil granulocytes, basophils, and T-cells. IL-8 (72a.a.) has a 5-10-fold higher activity on neutrophil activation, compared to IL-8 (77a.a.). IL-8 is often associated with inflammation and has been cited as a proinflammatory mediator in gingivitis and psoriasis. |
