產品詳情
簡單介紹：
重組人成纖維細胞生長因子13蛋白與其它公司提供的重組蛋白不同，rHuFGF-13蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人成纖維細胞生長因子13蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人成纖維細胞生長因子13蛋白
| Synonyms | FHF-2 |
| Species | Human |
| Accession | Q92913 |
| GeneID | 2258 |
| Source | Escherichia coli. |
| Molecular Weight | 重組人成纖維細胞生長因子13蛋白Approximately 27.6 kDa, a single non-glycosylated polypeptide chain containing 245 amino acids. |
| Quantity | 5μg/20μg/1000μg |
| AA Sequence | MAAAIASSLI RQKRQARERE KSNACKCVSS PSKGKTSCDK NKLNVFSRVK LFGSKKRRRR RPEPQLKGIV TKLYSRQGYH LQLQADGTID GTKDEDSTYT LFNLIPVGLR VVAIQGVQTK LYLAMNSEGY LYTSELFTPE CKFKESVFEN YYVTYSSMIY RQQQSGRGWY LGLNKEGEIM KGNHVKKNKP AAHFLPKPLK VAMYKEPSLH DLTEFSRSGS GTPTKSRSVS GVLNGGKSMS HNEST |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組人成纖維細胞生長因子13蛋白Test in process. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris, pH 8.5, 500 mM NaCl. |
| Endotoxin | Less than 1 EU/μg of rHuFGF-13 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | 重組人成纖維細胞生長因子13蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人成纖維細胞生長因子13蛋白 | |
| SDS-PAGE |
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| Reference |
1. Gerhard DS, Wagner L, Feingold EA, et al. 2004. Genome Res. 14:2121-7. 2. Wittmack EK, Rush AM, Craner MJ, et al. 2004. J Neurosci. 24:6765-75. 3. Smallwood PM, Munoz-Sanjuan I, Tong P, et al. 1996. Proc Natl Acad Sci U S A. 93:9850-7. 4. Olsen SK, Garbi M, Zampieri N, et al. 2003. J Biol Chem. 278:34226-36. 5. Hartung H, Feldman B, Lovec H, et al. 1997. Mech Dev. 64:31-9. |
| Background | Human FGF-13 encoded by the FGF13 gene, belongs to the FGF-11 subfamily which has four members FGF-11 to FGF-14. These four members were initially referred to as fibroblast growth factor homologous factors (FHFs) because they have high sequence identity with the other FGFs but did not activate FGF receptors (FGFRs) and were not generally considered members of the FGF family. FGF-13 plays a crucial role in neuron polarization and migration in the cerebral cortex. In murine FGF-13 RNA was detected in developing central nervous system in cells, and was also found throughout the peripheral nervous system. |
