產品詳情
簡單介紹：
重組鼠單核細胞趨化蛋白-5 / CCL12蛋白與其它公司提供的重組蛋白不同，rMuMCP-5/CCL12蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組鼠單核細胞趨化蛋白-5 / CCL12蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組鼠單核細胞趨化蛋白-5 / CCL12蛋白
| Synonyms | MCP-1-related Chemokine, Small-inducible Cytokine A12 |
| Species | Murine |
| Accession | Q62401 |
| GeneID | 20293 |
| Source | 重組鼠單核細胞趨化蛋白-5 / CCL12蛋白Escherichia coli. |
| Molecular Weight | Approximately 9.3 kDa, a single non-glycosylated polypeptide chain containing 82 amino acids. |
| Quantity | 5μg/20μg/1000μg |
| AA Sequence | GPDAVSTPVT CCYNVVKQKI HVRKLKSYRR ITSSQCPREA VIFRTILDKE ICADPKEKWV KNSINHLDKT SQTFILEPSC LG |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組鼠單核細胞趨化蛋白-5 / CCL12蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-50 ng/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/μg of rMuMCP-5/CCL12 as determined by LAL method. |
| Reconstitution | 重組鼠單核細胞趨化蛋白-5 / CCL12蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組鼠單核細胞趨化蛋白-5 / CCL12蛋白 | |
| Reference |
1. Sarafi MN, Garcia-Zepeda EA, MacLean JA, et al. 1997. J Exp Med. 185:99-109. 2. Jia GQ, Gonzalo JA, Lloyd C, et al. 1996. J Exp Med. 184:1939-51. |
| Background | Murine CCL12 is belonging to the CC chemokine family. It shows 66 % amino acid sequence identity to human MCP-1. CCL12 has been shown to be a potent chemoattractant for monocytes and lymphocytes but not neutrophils. At high concentrations, it will also chemoattract eosinophils. CCL12 can induce a calcium flux in human HEK-293 cells transfected with murine CCR2, but can not induce a calcium flux in HEK-293 cells transfected with CCR1, CCR3, or CCR5. That shows the functional receptor of CCL12 is CCR2. |
